Transient gene expression in shoot apical meristems of sugarbeet seedlings after particle bombardment
نویسندگان
چکیده
منابع مشابه
Meristems in the Movies: Live Imaging as a Tool for Decoding Intercellular Signaling in Shoot Apical Meristems
The shoot apical meristem (SAM) is the source of all postembryonic aerial organs in plants. Because of their central importance in plant development, SAMs have been studied for more than 100 years and have also become a major focus of molecular genetic studies. These studies indicate that signaling between cells is important in the maintenance of SAM size and structure and for the positioning a...
متن کاملMeristems in the movies: live imaging as a tool for decoding intercellular signaling in shoot apical meristems.
The shoot apical meristem (SAM) is the source of all postembryonic aerial organs in plants. Because of their central importance in plant development, SAMs have been studied for more than 100 years and have also become a major focus of molecular genetic studies. These studies indicate that signaling between cells is important in the maintenance of SAM size and structure and for the positioning a...
متن کاملMechanical stress contributes to the expression of the STM homeobox gene in Arabidopsis shoot meristems
The role of mechanical signals in cell identity determination remains poorly explored in tissues. Furthermore, because mechanical stress is widespread, mechanical signals are difficult to uncouple from biochemical-based transduction pathways. Here we focus on the homeobox gene SHOOT MERISTEMLESS (STM), a master regulator and marker of meristematic identity in Arabidopsis. We found that STM expr...
متن کاملMolecular cloning and characterization of genes expressed in shoot apical meristems.
The above-ground portion of a plant develops from the shoot apical meristem. An abundant source of apical meristems was obtained from cauliflower heads. Meristematic cDNAs were identified by differential screening and used to isolate corresponding Arabidopsis thaliana genes. Transcriptional promoters from Arabidopsis clones were fused to the beta-glucuronidase (GUS) reporter gene and introduced...
متن کاملOptimization of Transient Expression of uidA Gene in Androgenic Embryos of Wheat (Triticum aestivum L. cv. Falat) via Particle Pombardment
Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 1995
ISSN: 0022-0957,1460-2431
DOI: 10.1093/jxb/46.10.1625